Peroxidase 来源于辣根
Peroxidase from horseradish
Type VI, essentially salt-free, lyophilized powder, 250-330 units/mg solid （using pyrogallol） DOWNLOAD MSDS （PDF）

别名: Donor:hydrogen-peroxide oxidoreductase Horseradish peroxidase

CAS号 9003-99-0
EINECS 编号 232-668-6
Enzyme Commission （EC） Number 1.11.1.7 （ BRENDA | IUBMB ）
MDL number MFCD00071339
Related Categories 1.11.x.x Peroxide as receptor, 1.x.x.x Oxidoreductases, Analytical Enzymes, Application Index, Biochemicals and Reagents,
Carbohydrate-active Enzymes, Cell Biology, Cell Signaling and Neuroscience, Cell Stress, Diagnostic and Analytical Enzymes, Enzyme Class Index, Enzymes, Enzymes for Diagnostic Kit Manufacturing, Enzymes, Inhibitors, and Substrates, Glycoanalytical Enzymes, Glycobiology, Immunolabeling, Nitric Oxide and Cell Stress, Oxidative Stress Enzymes, Peroxidase, Peroxidase Enzymes and Reagents, Post-Translational Modification, Proteomics, Unmodified Horseradish Peroxidase （HRP）
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Quality Level PREMIUM
type Type VI
form essentially salt-free, lyophilized powder
absorbance ratio RZ 2.5-4.0
storage temp. 2-8°C
产品名称
Frequently Asked Questions
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Analysis Note
The RZ （Reinheitszahl） is the absorbance ratio A403/A275 determined at 0.5-1.0 mg/ml in deionized water. It is a measure of hemin content, not enzymatic activity. Even preparations with high RZ may have low enzymatic activity.

Other Notes
View more information on peroxidase at www.sigma-aldrich.com/enzymeexplorer.

Unit Definition
One pyrogallol unit will form 1.0 mg purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.

General description
Horseradish peroxidase is isolated from horseradish roots （Amoracia rusticana） and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme. Its molecular weight （~44 kDa） includes the polypeptide chain （33,890 Daltons）, hemin plus Ca2+ （~700 Daltons）, and carbohydrate （~9,400 Daltons）. At least seven isozymes of HRP exist. The isoelectric point for horseradish peroxidase isozymes ranges from 3.0 - 9.0.